5-Amino-1MQ

Inventory Logic

Why 5mg Is Deliberately Too Small for In Vivo Work

At the Neelakantan 2018 rodent dose of 20mg/kg/day, a single 250g mouse consumes 5mg in one day — so this vial cannot support a meaningful in vivo endpoint. That is not a limitation, it is the point. The 5mg format is sized for in vitro characterization that happens before any in vivo protocol: solubility screening, cell-culture dose-response, enzyme kinetics, and assay-compatibility testing.

Well-Plate Math

Hundreds of Experiments on a Single Vial

A 24-well adipocyte differentiation plate at 10 μM final 5-Amino-1MQ concentration consumes roughly 5-10 μg total per plate (200 μL/well × 24 wells × 10 μM × ~200 Da MW). Starting from 5mg inventory that equals 500-1000 plates of adipocyte work, or many thousand individual well-level observations. For enzyme kinetic experiments on purified recombinant NNMT, the throughput is higher still.

Try-Before-Scale Format

The Pilot Vial as Workflow Choice

Research groups new to 5-Amino-1MQ often need to verify that the compound dissolves cleanly in their vehicle, hits expected IC50 in their specific assay, and produces stable activity across their working concentration range before committing to 50mg-scale inventory. The 5mg format is intentionally sized to support that evaluation phase without overcommitting. Once the internal workflow is validated, scaling to larger formats is straightforward.